Agar plates are the standard solid support material for growing microorganisms. Microbial growth media contains nutrients and an energy source to fuel the microbes as they grow, and agar to keep the media in a semi–solid, gel–like state.
On solid media, a single microbe will grow and divide to produce a "colony," a spot of identical descendants. Different types of microbes produce colonies with different characteristics—shape, color, texture—which help microbiologists determine if a culture is pure, or identify the types of microbes in a mixed sample.
A number of biological supply companies sell pre–made plates, but making your own is much less expensive. With a little practice, you will find that it is very easy to make your own plates, and you will have the added flexibility of being able to customize recipes to suit your needs.Agar plates are the standard solid support material for growing microorganisms. Microbial growth media contains nutrients and an energy source to fuel the microbes as they grow, and agar to keep the media in a semi–solid, gel–like state.
On solid media, a single microbe will grow and divide to produce a "colony," a spot of identical descendants. Different types of microbes produce colonies with different characteristics—shape, color, texture—which help microbiologists determine if a culture is pure, or identify the types of microbes in a mixed sample.
A number of biological supply companies sell pre–made plates, but making your own is much less expensive. With a little practice, you will find that it is very easy to make your own plates, and you will have the added flexibility of being able to customize recipes to suit your needs.Agar plates are the standard solid support material for growing microorganisms. Microbial growth media contains nutrients and an energy source to fuel the microbes as they grow, and agar to keep the media in a semi–solid, gel–like state.
On solid media, a single microbe will grow and divide to produce a "colony," a spot of identical descendants. Different types of microbes produce colonies with different characteristics—shape, color, texture—which help microbiologists determine if a culture is pure, or identify the types of microbes in a mixed sample.
A number of biological supply companies sell pre–made plates, but making your own is much less expensive. With a little practice, you will find that it is very easy to make your own plates, and you will have the added flexibility of being able to customize recipes to suit your needs.
OBJECTIVE
To prepare sterile nutrient agar for culturing microorganism
MATERIALS AND REAGENTS
Commercial nutrient agar
Balanace
Distilled water
Scott Bottles
PROCEDURE
(refer to lab manual)
RESULT
DISCUSSION
An autoclaved is a a strong heated container used for chemical reactions and other processes using high pressures and temperatures, e.g. steam sterilization.Autoclaves work in a similar way, but they're typically used in a more extreme form of cooking: to blast the bugs and germs on things with steam long enough to sterilize them. The extra pressure in an autoclave means that water boils at a temperature higher than its normal boiling point—roughly 20°C hotter—so it holds and carries more heat and kills microbes more effectively. A lengthy blast of high-pressure steam is much more effective at penetrating and sterilizing things than a quick wipe in ordinary hot water.When microbiological media has been made, it still has to be sterilized because of microbial contamination from air, glassware, hands, etc. Within a few hours there will be thousands of bacteria reproducing in the media so it has to be sterilized quickly before the microbes start using the nutrients up. The sterilization process is a 100% kill, and guarantees that the medium will stay sterile UNLESS exposed to contaminants by less that adequate aseptic technique to exposure to air.
RESULT
DISCUSSION
An autoclaved is a a strong heated container used for chemical reactions and other processes using high pressures and temperatures, e.g. steam sterilization.Autoclaves work in a similar way, but they're typically used in a more extreme form of cooking: to blast the bugs and germs on things with steam long enough to sterilize them. The extra pressure in an autoclave means that water boils at a temperature higher than its normal boiling point—roughly 20°C hotter—so it holds and carries more heat and kills microbes more effectively. A lengthy blast of high-pressure steam is much more effective at penetrating and sterilizing things than a quick wipe in ordinary hot water.When microbiological media has been made, it still has to be sterilized because of microbial contamination from air, glassware, hands, etc. Within a few hours there will be thousands of bacteria reproducing in the media so it has to be sterilized quickly before the microbes start using the nutrients up. The sterilization process is a 100% kill, and guarantees that the medium will stay sterile UNLESS exposed to contaminants by less that adequate aseptic technique to exposure to air.
CONCLUSION
the preparation of nutrient agar for culturing microorganism is done by using autoclaving technique in oder the material to be sterilized, it is very important to ensure that all of thetrapped air is removed.
Proper autoclaved treatment will inactive all fungi,bacteria,viruses,and also bacterial spores,which can be quite resistant,if not necessarily eliminate all prions.
REFERENCE
http://www.microbiologyonline.org.uk/teachers/preparation-of-media-and-cultures
http://www.ruf.rice.edu/~bioslabs/bios318/318manual.htm
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